Title : The extracellular patch clamp: a method for resolving currents through individual open channels in biological membranes - Neher_1978_Pflugers.Arch_375_219 |
Author(s) : Neher E , Sakmann B , Steinbach JH |
Ref : Pflugers Arch , 375 :219 , 1978 |
Abstract :
The current contributions of individual ionic channels can be measured by electrically isolating a small patch of membrane. To do this, the tip of a small pipette is brought into close contact with an enzymatically cleaned membrane of a hypersensitive amphibian or mammalian muscle fiber. Current flowing through the pipette is measured. If the pipette contains cholinergic agonist at mu-molar concentrations, square pulse current waveforms can be observed which represent the activation of individual acetylcholine-receptor channels. The square pulses have amplitudes of 1 to 3 pA and durations of 10--100ms. In order to obtain the necessary resolution, a delicate compromise had to be found between different experimental parameters. Pipettes with 1--3 micrometer internal diameter and a steep final taper had to be used, extensive enzyme treatment was necessary, and conditions had be to found in which channels open at a relatively low frequency. |
PubMedSearch : Neher_1978_Pflugers.Arch_375_219 |
PubMedID: 567789 |
Neher E, Sakmann B, Steinbach JH (1978)
The extracellular patch clamp: a method for resolving currents through individual open channels in biological membranes
Pflugers Arch
375 :219
Neher E, Sakmann B, Steinbach JH (1978)
Pflugers Arch
375 :219