Title : Gene trap insertional mutagenesis in mice: new vectors and germ line mutations in two novel genes - Neilan_1999_Transgenic.Res_8_451 |
Author(s) : Neilan EG , Barsh GS |
Ref : Transgenic Res , 8 :451 , 1999 |
Abstract :
Insertional mutagenesis based on gene trap vectors that capture endogenous splice sites is a promising tool for functional genomics. Several groups have proposed large-scale gene trap screens, but questions remain as to the type of vectors and their design. We report a set of plasmid-encoded gene trap vectors and the disruption of two novel genes. Our results include a comparison of the relative gene trapping efficiencies of two different splice acceptor sequences in ES cells and an analysis of the structure of several gene trap insertions. |
PubMedSearch : Neilan_1999_Transgenic.Res_8_451 |
PubMedID: 10767988 |
Gene_locus related to this paper: mouse-f172a |
Gene_locus | mouse-f172a |
Neilan EG, Barsh GS (1999)
Gene trap insertional mutagenesis in mice: new vectors and germ line mutations in two novel genes
Transgenic Res
8 :451
Neilan EG, Barsh GS (1999)
Transgenic Res
8 :451