| Title : Expression of a highly antigenic and native-like folded extracellular domain of the human alpha1 subunit of muscle nicotinic acetylcholine receptor, suitable for use in antigen specific therapies for Myasthenia Gravis - Niarchos_2013_PLoS.One_8_e84791 |
| Author(s) : Niarchos A , Zouridakis M , Douris V , Georgostathi A , Kalamida D , Sotiriadis A , Poulas K , Iatrou K , Tzartos SJ |
| Ref : PLoS ONE , 8 :e84791 , 2013 |
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Abstract :
We describe the expression of the extracellular domain of the human alpha1 nicotinic acetylcholine receptor (nAChR) in lepidopteran insect cells (i-alpha1-ECD) and its suitability for use in antigen-specific therapies for Myasthenia Gravis (MG). Compared to the previously expressed protein in P. pastoris (y-alpha1-ECD), i-alpha1-ECD had a 2-fold increased expression yield, bound anti-nAChR monoclonal antibodies and autoantibodies from MG patients two to several-fold more efficiently and resulted in a secondary structure closer to that of the crystal structure of mouse alpha1-ECD. Our results indicate that i-alpha1-ECD is an improved protein for use in antigen-specific MG therapeutic strategies. |
| PubMedSearch : Niarchos_2013_PLoS.One_8_e84791 |
| PubMedID: 24376846 |
Niarchos A, Zouridakis M, Douris V, Georgostathi A, Kalamida D, Sotiriadis A, Poulas K, Iatrou K, Tzartos SJ (2013)
Expression of a highly antigenic and native-like folded extracellular domain of the human alpha1 subunit of muscle nicotinic acetylcholine receptor, suitable for use in antigen specific therapies for Myasthenia Gravis
PLoS ONE
8 :e84791
Niarchos A, Zouridakis M, Douris V, Georgostathi A, Kalamida D, Sotiriadis A, Poulas K, Iatrou K, Tzartos SJ (2013)
PLoS ONE
8 :e84791