Title : Inhibition of human plasma and serum butyrylcholinesterase (EC 3.1.1.8) by alpha-chaconine and alpha-solanine - Nigg_1996_Fundam.Appl.Toxicol_33_272 |
Author(s) : Nigg HN , Ramos LE , Graham EM , Sterling J , Brown S , Cornell JA |
Ref : Fundamental & Applied Toxicology , 33 :272 , 1996 |
Abstract :
The purpose of these experiments was to determine the reversibility of alpha-chaconine and alpha-solanine inhibition of human plasma butyrylcholinesterase (BCHE). For the substrate alpha-naphthylacetate, optimal assay conditions were 0.50 M sodium phosphate buffer and a substrate concentration of 3-5 x 10(-4) M. Dibucaine (1 x 10(-5) M) indicated the usual phenotype for all subjects; alpha-chaconine and alpha-solanine at 2.88 x 10(-6) M inhibited BCHE about 70 and 50%, respectively. One- and 24-hr incubations at 1 x 10(-5) M with alpha-chaconine, alpha-solanine, paraoxon, eserine, and ethanol yielded reversible inhibition with dilution except for paraoxon. Twenty-four-hour dialyses of incubations showed no inhibition except for paraoxon. PAGE enzyme activity gels of 1- and 24-hr incubations also showed no inhibition except for paraoxon. alpha-Chaconine and alpha-solanine are reversible inhibitors of human butyrylcholinesterase. At estimated tissue levels, alpha-chaconine, alpha-solanine, and solanidine inhibited BCHE 10-86%. In assays which combined alpha-chaconine, alpha-solanine, and solanidine, inhibition of BCHE was less than additive. No inhibition of albumin alpha-naphthylacetate esterase (an arylesterase) was noted with any inhibitor. The importance of these data to adverse toxicological effects of potato alkaloids is discussed. |
PubMedSearch : Nigg_1996_Fundam.Appl.Toxicol_33_272 |
PubMedID: 8921346 |
Inhibitor | Solanidine Solanine |
Nigg HN, Ramos LE, Graham EM, Sterling J, Brown S, Cornell JA (1996)
Inhibition of human plasma and serum butyrylcholinesterase (EC 3.1.1.8) by alpha-chaconine and alpha-solanine
Fundamental & Applied Toxicology
33 :272
Nigg HN, Ramos LE, Graham EM, Sterling J, Brown S, Cornell JA (1996)
Fundamental & Applied Toxicology
33 :272