Nomura_2000_Pestic.Biochem.Physiol_66_73

Heredity of modified acetylcholinesterase (AChE) of the green rice leafhopper (Nephotettix cincticeps Uhler) was assessed by crossing experiments between different strains and by analyzing AChE activity of individual insect heads to both metolcarb and N-propyl metolcarb. The green rice leafhopper which shows resistance to metolcarb was found to possess different AChE isozymes, which show negatively correlated cross-resistance to N-propyl metolcarb. However, each insect possessed only one type of AChE isozyme. By crossing experiments between different strains of the green rice leafhopper, at least seven different AChE phenotypes were observed. The method for analyzing AChE of individual insect was found to be a useful tool for the genetic analysis of organophosphorus and carbamate insecticide resistance of the green rice leafhopper.