Odendall_2019_Mol.Biol.Cell__mbcE19030166

Mitochondria are unique organelles harboring two distinct membranes, the mitochondrial inner and outer membrane (MIM and MOM, respectively). Mitochondria comprise only a subset of metabolic pathways for the synthesis of membrane lipids, therefore most lipid species and their precursors have to be imported from other cellular compartments. One of such import processes is mediated by the ER mitochondria encounter structure (ERMES) complex. Both mitochondrial membranes surround the hydrophilic intermembrane space (IMS). Therefore additional systems are required that shuttle lipids between MIM and MOM. Recently, we identified the IMS protein Mcp2 as a high-copy suppressor for cells that lack functional ERMES complex. To understand better, how mitochondria facilitate transport and biogenesis of lipids, we searched for genetic interactions of this suppressor. We found, that MCP2 has a negative genetic interaction with the gene TGL2 encoding a neutral lipid hydrolase. We show that this lipase is located in the intermembrane space of mitochondria and is imported via the Mia40 disulfide relay system. Furthermore, we show a positive genetic interaction of double deletion of MCP2 and PSD1, the gene encoding the enzyme that synthesizes the major amount of cellular phosphatidylethanolamine. Finally, we demonstrate that the nucleotide binding motifs of the predicted atypical kinase Mcp2 are required for its proper function. Taken together, our data suggest that Mcp2 is involved in mitochondrial lipid metabolism and a rise of this involvement by overexpression suppresses loss of ERMES.