Olalekan_2014_Free.Radic.Biol.Med_71C_99

Reference

Title : Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster - Olalekan_2014_Free.Radic.Biol.Med_71C_99
Author(s) : Olalekan Abolaji A , Paul Kamdem J , Henrique Lugokenski T , Kalar Nascimento T , Pansera Waczuk E , Olatunde Farombi E , da Silva Loreto EL , Teixeira Rocha JB
Ref : Free Radic Biol Med , 71C :99 , 2014
Abstract :

4-Vinylcyclohexene (VCH) is a dimer of 1,3-butadiene produced as a by-product of pesticides, plastic, rubber, flame retardants, and tire production. Although, several studies have reported the ovotoxicity of VCH, information on a possible involvement of oxidative stress in the toxicity of this occupational chemical is scarce. Hence, this study was carried out to investigate further possible mechanisms of toxicity of VCH with a specific emphasis on oxidative stress using a Drosophila melanogaster model. D. melanogaster (both genders) of 1 to 3 days old were exposed to different concentrations of VCH (10microM-1mM) in the diet for 5 days. Subsequently, the survival and negative geotaxis assays and the quantification of reactive oxygen species (ROS) generation were determined. In addition, we evaluated RT-PCR expressions of selected oxidative stress and antioxidant mRNA genes (HSP27, 70, and 83, SOD, Nrf-2, MAPK2, and catalase). Furthermore, catalase, glutathione-S-transferase (GST), delta aminolevulinic acid dehydratase (delta-ALA-D), and acetylcholinesterase (AChE) activities were determined. VCH exposure impaired negative geotaxic behavior and induced the mRNA of SOD, Nrf-2, and MAPK2 genes expressions. There were increases in catalase and ROS production, as well as inhibitions of GST, delta-ALA-D, and AChE activities (P<0.05). Our results suggest that the VCH mechanism of toxicity is associated with oxidative damage, as evidenced by the alteration in the oxidative stress-antioxidant balance, and possible neurotoxic consequences due to decreased AChE activity, and impairments in negative geotaxic behavior. Thus, we conclude that D. melanogaster is a useful model for investigating the toxicity of VCH exposure, and here, we have provided further insights on the mechanism of VCH-induced toxicity.

PubMedSearch : Olalekan_2014_Free.Radic.Biol.Med_71C_99
PubMedID: 24681254

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Citations formats

Olalekan Abolaji A, Paul Kamdem J, Henrique Lugokenski T, Kalar Nascimento T, Pansera Waczuk E, Olatunde Farombi E, da Silva Loreto EL, Teixeira Rocha JB (2014)
Involvement of oxidative stress in 4-vinylcyclohexene-induced toxicity in Drosophila melanogaster
Free Radic Biol Med 71C :99

Olalekan Abolaji A, Paul Kamdem J, Henrique Lugokenski T, Kalar Nascimento T, Pansera Waczuk E, Olatunde Farombi E, da Silva Loreto EL, Teixeira Rocha JB (2014)
Free Radic Biol Med 71C :99