Oliva_2026_Chem.Biodivers_23_e71641

Alzheimer's disease (AD) is a neurodegenerative disorder associated with amyloid-beta (Abeta) aggregation, advanced glycation end products (AGEs), and oxidative stress. This study investigated the antiglycation, antioxidant, anti-amyloidogenic, and predicted drug-likeness properties of Pandanus odorifer (Forssk.) Kuntze. The methanolic crude extract showed high total flavonoid content (121.7 +/- 2.50 mg QE/g), moderate total phenolic content (16.61 +/- 1.50 mg GAE/g), and strong AGE inhibition (IC(50) = 57.30 microg/mL), with moderate radical scavenging activity. Chromatographic separation yielded syringaresinol (1), dehydrovomifoliol (2), and blumenol C glucoside (3), identified through spectroscopic analysis and comparison with published data. In the Thioflavin T assay, compounds 1-3 reduced Abeta-associated fluorescence, with compound 3 producing the largest reduction (71.20% +/- 4.42%) under the tested conditions. Molecular docking suggested interactions with Abeta42 oligomers and AD-related enzymes, particularly acetylcholinesterase and beta-secretase, while in silico prediction indicated acceptable drug-likeness without Lipinski violations. Overall, the findings identify P. odorifer as a source of constituents with antiglycation activity and preliminary Abeta-associated ThT responses that warrant confirmation using orthogonal biophysical and biological assays.