Oliveira_2013_Ticks.Tick.Borne.Dis_4_469

Reference

Title : Proteome of Rhipicephalus sanguineus tick saliva induced by the secretagogues pilocarpine and dopamine - Oliveira_2013_Ticks.Tick.Borne.Dis_4_469
Author(s) : Oliveira CJ , Anatriello E , de Miranda-Santos IK , Francischetti IM , Sa-Nunes A , Ferreira BR , Ribeiro JM
Ref : Ticks Tick Borne Dis , 4 :469 , 2013
Abstract :

One dimensional gel electrophoresis was used to separate proteins from the saliva of Rhipicephalus sanguineus female ticks fed on rabbits. Gel slices were subjected to tryptic digestion and analyzed by reversed-phase HPLC followed by MS/MS analysis. The data were compared to a database of salivary proteins of the same tick and to the predicted proteins of the host. Saliva was obtained by either pilocarpine or dopamine stimulation of partially fed ticks. Electrophoretic separations of both yielded products that were identified by mass spectrometry, although the pilocarpine-derived sample was of much better quality. The majority of identified proteins were of rabbit origin, indicating the recycling of the host proteins in the tick saliva, including hemoglobin, albumin, haptoglobin, transferring, and a plasma serpin. The few proteins found that were previously associated with parasitism and blood feeding include 2 glycine-rich, cement-like proteins, 2 lipocalins, and a thyropin protease inhibitor. Among other of the 19 tick proteins identified, albeit with undefined roles, were SPARC and cyclophilin A. This catalog provides a resource that can be mined for secreted molecules that play a role in tick-host interactions.

PubMedSearch : Oliveira_2013_Ticks.Tick.Borne.Dis_4_469
PubMedID: 24029695

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Citations formats

Oliveira CJ, Anatriello E, de Miranda-Santos IK, Francischetti IM, Sa-Nunes A, Ferreira BR, Ribeiro JM (2013)
Proteome of Rhipicephalus sanguineus tick saliva induced by the secretagogues pilocarpine and dopamine
Ticks Tick Borne Dis 4 :469

Oliveira CJ, Anatriello E, de Miranda-Santos IK, Francischetti IM, Sa-Nunes A, Ferreira BR, Ribeiro JM (2013)
Ticks Tick Borne Dis 4 :469