| Title : Mechanism of Rhinella icterica (Spix, 1824) toad poisoning using in vitro neurobiological preparations - Oliveira_2018_Neurotoxicol_65_264 |
| Author(s) : Oliveira RS , Leal AP , Ogata B , Moreira de Almeida CG , Dos Santos DS , Lorentz LH , Moreira CM , de Castro Figueiredo Bordon K , Arantes EC , Dos Santos TG , Dal Belo CA , Vinade L |
| Ref : Neurotoxicology , 65 :264 , 2018 |
| Abstract : The biological activity of Rhinella icterica toxic secretion (RITS) was evaluated on chick neuromuscular junctions, rat hearts tissue and mice hippocampal slices. At chick biventer cervicis preparation, RITS (5, 10 and 20mug/mL) produced a concentration-independent irreversible neuromuscular blockade, which was preceded by a transitory increase of muscle twitch tension with the lowest concentration, in 120min recordings. In this set of experiments, RITS incubation partially prevented the curare neuromuscular blockade. The assessment of chick biventer cervicis muscle acetylcholinesterase (AChE) in the presence of RITS showed a significant inhibition of the enzyme, similarly to neostigmine. The incubation of muscles with digoxin or ouabain mimicked the poison activity by increasing the amplitude of the twitches followed by a progressive depression of the muscle strength. In addition, RITS demonstrated a digitalic-like activity, by inhibiting significantly the cardiac Na(+), K(+)-ATPase. When the central nervous system was accessed, RITS induced an increase in the cell viability, in the lowest concentration. In addition, the poison protected slices subject to oxygen/glucose deprivation. Altogether, these data indicate that the poisonous extract of R. icterica is able to interfere with peripheral and central neurotransmission, probably due to a direct interaction with AChE, calcium channels and Na(+), K(+)-ATPase. A further investigation upon the poison toxic components will unveil the components involved in such a pharmacological activity and the potential biotechnological application of this poison. |
| ESTHER : Oliveira_2018_Neurotoxicol_65_264 |
| PubMedSearch : Oliveira_2018_Neurotoxicol_65_264 |
| PubMedID: 29174112 |
Oliveira RS, Leal AP, Ogata B, Moreira de Almeida CG, Dos Santos DS, Lorentz LH, Moreira CM, de Castro Figueiredo Bordon K, Arantes EC, Dos Santos TG, Dal Belo CA, Vinade L (2018)
Mechanism of Rhinella icterica (Spix, 1824) toad poisoning using in vitro neurobiological preparations
Neurotoxicology
65 :264
Oliveira RS, Leal AP, Ogata B, Moreira de Almeida CG, Dos Santos DS, Lorentz LH, Moreira CM, de Castro Figueiredo Bordon K, Arantes EC, Dos Santos TG, Dal Belo CA, Vinade L (2018)
Neurotoxicology
65 :264