Onur_2025_Bioorg.Chem_164_108916

This study explores the multifaceted pharmacological potential of novel p-cresol-triazole hybrid compounds as candidates for complex diseases. A series of 1,2,3-triazole-p-cresol hybrids was synthesized via Cu(I)-catalyzed click chemistry and structurally confirmed using comprehensive spectroscopic methods and X-ray crystallography. The compounds were evaluated for their biological properties, revealing significant acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activities, with certain derivatives outperforming the reference drugs galantamine and donepezil. Anticancer assays identified compounds with electron-withdrawing substituents showing potent and selective cytotoxicity against the Caco-2 colon cancer cell line compared to a normal fibroblast cell line. Furthermore, antioxidant evaluations highlighted several compounds as effective radical scavengers. In silico ADMET predictions confirmed that all compounds possess favorable pharmacokinetic profiles and adhere to Lipinski's Rule of Five. Molecular docking studies provided insights into the binding interactions with AChE/BuChE active sites, corroborating the experimental findings. These results position p-cresol-triazole hybrids as promising multifunctional leads for further preclinical investigation in the context of neurodegenerative diseases and oncology.