| Title : Immature megakaryocytes undergo apoptosis in the absence of thrombopoietin - Osada_1999_Exp.Hematol_27_131 |
| Author(s) : Osada M , Komeno T , Todokoro K , Takizawa M , Kojima H , Suzukawa K , Ninomiya H , Abe T , Nagasawa T |
| Ref : Experimental Hematology , 27 :131 , 1999 |
|
Abstract :
We examined withdrawal effects of recombinant mouse Tpo (rm-Tpo) on the apoptosis of mature and immature megakaryocytes in in vitro experiments. Apoptotic megakaryocytes were detected by double staining for acetylcholinesterase and by the TdT-mediated dUTP-biotin nick end labeling (TUNEL) method. When the purified mature megakaryocytes were cultured with or without rm-Tpo, the numbers of viable megakaryocytes, apoptotic megakaryocytes, and megakaryocytes with cytoplasmic processes were not significantly different between the two groups. In contrast, purified immature megakaryocytes underwent apoptosis when rm-Tpo was absent from the culture system. Murine bone marrow cells were cultured with rm-Tpo (50 U/mL) on days 1-7 to generate immature megakaryocytes and subsequently were cultured with different concentrations of rm-Tpo (0-50 U/mL) on days 8-14. The number of viable megakaryocytes was decreased and that of apoptotic megakaryocytes was increased by rm-Tpo in a dose-dependent manner. These results indicated a clear relation between the rm-Tpo level and the apoptosis of immature megakaryocytes. |
| PubMedSearch : Osada_1999_Exp.Hematol_27_131 |
| PubMedID: 9923451 |
Osada M, Komeno T, Todokoro K, Takizawa M, Kojima H, Suzukawa K, Ninomiya H, Abe T, Nagasawa T (1999)
Immature megakaryocytes undergo apoptosis in the absence of thrombopoietin
Experimental Hematology
27 :131
Osada M, Komeno T, Todokoro K, Takizawa M, Kojima H, Suzukawa K, Ninomiya H, Abe T, Nagasawa T (1999)
Experimental Hematology
27 :131