| Title : Suppression of neutral cholesterol ester hydrolase activity by antisense DNA of hormone-sensitive lipase - Osuga_1997_Biochem.Biophys.Res.Commun_233_655 |
| Author(s) : Osuga J , Ishibashi S , Shimano H , Inaba T , Kawamura M , Yazaki Y , Yamada N |
| Ref : Biochemical & Biophysical Research Communications , 233 :655 , 1997 |
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Abstract :
In order to investigate the role of the hormone-sensitive lipase (HSL) gene in the activity of neutral cholesterol ester hydrolase (NCEH) from a molecular perspective, Chinese hamster ovary (CHO) cells were transfected with rat antisense hormone-sensitive lipase cDNA, and three different cell lines, designated as anti-HSL, were established. NCEH activity in anti-HSL cells was reduced to approximately 50% of that in control CHO cells. The concentration of cellular esterified cholesterol increased and the concentration of free cholesterol decreased in the anti-HSL cell lines. These results suggest that the HSL gene has a function of NCEH as well. |
| PubMedSearch : Osuga_1997_Biochem.Biophys.Res.Commun_233_655 |
| PubMedID: 9168908 |
Osuga J, Ishibashi S, Shimano H, Inaba T, Kawamura M, Yazaki Y, Yamada N (1997)
Suppression of neutral cholesterol ester hydrolase activity by antisense DNA of hormone-sensitive lipase
Biochemical & Biophysical Research Communications
233 :655
Osuga J, Ishibashi S, Shimano H, Inaba T, Kawamura M, Yazaki Y, Yamada N (1997)
Biochemical & Biophysical Research Communications
233 :655