Oydvin_2005_Eur.J.Pharmacol_516_227

Reference

Title : Pre-junctional effects of oximes on [3H]-acetylcholine release in rat hippocampal slices during soman intoxication - Oydvin_2005_Eur.J.Pharmacol_516_227
Author(s) : Oydvin OK , Tanso R , Aas P
Ref : European Journal of Pharmacology , 516 :227 , 2005
Abstract :

In this study, the non-reactivating effects of oximes in the hippocampus of the rat are investigated. The potassium (51 mM) evoked release of [(3)H]-acetylcholine and the liberation of [(3)H]-choline were determined in hippocampal slices following in vitro exposure to soman and five oximes (toxogonin, HI-6, HLo-7, P2S and 2-PAM) in separate experiments by superfusion. In the absence of soman, toxogonin and HLo-7 in particular induced a concentration dependent significant increase in the evoked release of [(3)H]-acetylcholine. There was also a significant effect of HI-6, but the effect was much smaller. Two pralidoxime salts, P2S (methanesulfonate salt) and 2-PAM (methiodide salt), had similar but lower effects that were only observed at relatively high concentrations. Experiments performed following complete inhibition of the acetylcholinesterase activity by soman (1.0 microM) showed that HI-6 and HLo-7 induced a significant decrease in the potassium-evoked release of [(3)H]-acetylcholine, while the liberation of [(3)H]-choline increased. Toxogonin, P2S and 2-PAM did not reduce significantly the evoked release of [(3)H]-acetylcholine. Only limited reactivation of the acetylcholinesterase activity was observed in superfusion experiments with toxogonin, HI-6, P2S and 2-PAM following exposure of hippocampal slices to soman. However, HLo-7 was proved to be relatively more effective in reactivating the acetylcholinesterase activity at high concentrations (50 and 200 microM). The acetylcholinesterase activity was reactivated to approximately 12% and 40% of control, respectively. It is concluded that HI-6 and HLo-7 have important non-acetylcholinesterase reactivating properties following soman poisoning, as may be seen by the significant reduction in the evoked release of [(3)H]-acetylcholine effected by these oximes. HLo-7 is of particular interest in view of its ability to additionally improve reactivation of the acetylcholinesterase activity.

PubMedSearch : Oydvin_2005_Eur.J.Pharmacol_516_227
PubMedID: 15967427

Related information

Citations formats

Oydvin OK, Tanso R, Aas P (2005)
Pre-junctional effects of oximes on [3H]-acetylcholine release in rat hippocampal slices during soman intoxication
European Journal of Pharmacology 516 :227

Oydvin OK, Tanso R, Aas P (2005)
European Journal of Pharmacology 516 :227