| Title : Modulation of choline acetyltransferase synthesis by okadaic acid, a phosphatase inhibitor, and KN-62, a CaM kinase inhibitor, in NS-20Y neuroblastoma - Pahud_2001_Neurochem.Int_38_75 |
| Author(s) : Pahud G , Bontron S , Eder-Colli L |
| Ref : Neurochem Int , 38 :75 , 2001 |
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Abstract :
Choline-O-acetyltransferase (ChAT) is the enzyme which catalyses the biosynthesis of the neurotransmitter acetylcholine in cholinergic neurons. Here we show that in mouse cholinergic NS-20Y neuroblastoma cells cultured in the presence of either okadaic acid (serine/threonine phosphatases 1 and 2A inhibitor) or KN-62 (CaM kinase inhibitor) ChAT activity and mRNA either increased or decreased as a function of the drug concentration, respectively. After 24 h exposure, okadaic acid exerted a dramatic effect on cell morphology; cells became round and had no more neurites. On the contrary, KN-62 induced a slight morphological differentiation of the cells. The present results suggest that phosphatases 1 and 2A and CaM kinase could mediate regulation of ChAT gene expression. |
| PubMedSearch : Pahud_2001_Neurochem.Int_38_75 |
| PubMedID: 10913690 |
Pahud G, Bontron S, Eder-Colli L (2001)
Modulation of choline acetyltransferase synthesis by okadaic acid, a phosphatase inhibitor, and KN-62, a CaM kinase inhibitor, in NS-20Y neuroblastoma
Neurochem Int
38 :75
Pahud G, Bontron S, Eder-Colli L (2001)
Neurochem Int
38 :75