Palacio-Cortes_2017_Ecotoxicol.Environ.Saf_139_308

In-vivo effects of polybrominated diphenyl ethers (PBDEs) containing 3, 4 and 5 bromine atoms were tested on fourth-instar larvae of Chironomus sancticaroli (Diptera: Chironomidae) after 48h of exposure, by measuring the activity of the acetyl cholinesterase, alpha and beta esterases and glutathione S-transferase. The PBDE congeners 2,2',4-triBDE (BDE-17), 2,2',4,4'-tetraBDE (BDE-47) and 2,2',4,4',5-pentaBDE (BDE-99) were evaluated at 0.5, 1.0, 2.0 and 3.0ngmL-1. Acetyl cholinesterase activity decreased significantly (p<=0.05) at all evaluated concentrations of the three PBDE congeners, except for larvae exposed to BDE-17 at 1.0 and 2.0ngmL-1. The significant inhibition of acetyl cholinesterase activity ranged from 18% (BDE-47 at 0.5ngmL-1) to 72% (BDE-47 at 2.0ngmL-1). The enzymes alpha and beta esterase were also affected by the three congeners, reducing their activity from 14% (BDE-99 at 1.0ngmL-1) to 52% (BDE-47 at 2.0ngmL-1) and from 7% (BDE-99 at 2.0ngmL-1) to 34% (BDE-47 at 3.0ngmL-1) respectively. Substantial increments in glutathione S-transferase activity were similarly observed, varying from 138% (BDE-99 2.0 at ng mL-1) to 346% (BDE-17 at 1.0ngmL-1). DNA strand breaks were detected exclusively in larvae exposed to BDE-99 at 2.0 and 3.0ngmL-1 (H=11.7, p=0.019). These results showed that C. sancticaroli larvae were sensitive to the PBDEs treatments under the experimental conditions.