Title : Sensitive detection of butyrylcholinesterase activity based on a stimuli-responsive fluorescence reaction - Pang_2023_Spectrochim.Acta.A.Mol.Biomol.Spectrosc_299_122886 |
Author(s) : Pang Y , Ma Z , Song Q , Wang Z , Shi YE |
Ref : Spectrochim Acta A Mol Biomol Spectrosc , 299 :122886 , 2023 |
Abstract :
A fluorogenic reaction between the chelate of Mn(II)-citric acid and terephthalic acid (PTA) was discovered, which was carried out through heating the aqueous mixture of Mn(2+), citric acid and PTA. Detailed investigations indicated the reaction products were 2-hydroxyterephthalic acid (PTA-OH), which was attributed to the reaction between PTA and OH, formed by the triggering of Mn(II)-citric acid in the presence of dissolved O(2). PTA-OH showed a strong blue fluorescence, peaked at 420 nm, and the fluorescence intensity presented a sensitive response to pH of the reaction system. Based on these mechanisms, the fluorogenic reaction was used for the detection of butyrylcholinesterase activity, achieving a detection limit of 0.15 U/L. The detection strategy was successfully applied in human serum samples, and it was also extended for the detection of organophosphorus pesticides and radical scavengers. Such a facile fluorogenic reaction and its stimuli-responsive properties offered an effective tool for designing detection pathways in the fields of clinical diagnosis, environmental monitoring and bioimaging. |
PubMedSearch : Pang_2023_Spectrochim.Acta.A.Mol.Biomol.Spectrosc_299_122886 |
PubMedID: 37210854 |
Pang Y, Ma Z, Song Q, Wang Z, Shi YE (2023)
Sensitive detection of butyrylcholinesterase activity based on a stimuli-responsive fluorescence reaction
Spectrochim Acta A Mol Biomol Spectrosc
299 :122886
Pang Y, Ma Z, Song Q, Wang Z, Shi YE (2023)
Spectrochim Acta A Mol Biomol Spectrosc
299 :122886