| Title : Saturation mutagenesis in selected amino acids to shift Pseudomonas sp. acidic lipase Lip I.3 substrate specificity and activity - Panizza_2015_Chem.Commun.(Camb)_51_1330 |
| Author(s) : Panizza P , Cesarini S , Diaz P , Rodriguez Giordano S |
| Ref : Chem Commun (Camb) , 51 :1330 , 2015 |
|
Abstract :
Several Pseudomonas sp. CR611 Lip I.3 mutants with overall increased activity and a shift towards longer chain substrates were constructed. Substitution of residues Y29 and W310 by smaller amino acids provided increased activity on C18-substrates. Residues G152 and S154, modified to study their influence on interfacial activation, displayed a five and eleven fold increased activity. |
| PubMedSearch : Panizza_2015_Chem.Commun.(Camb)_51_1330 |
| PubMedID: 25482450 |
Panizza P, Cesarini S, Diaz P, Rodriguez Giordano S (2015)
Saturation mutagenesis in selected amino acids to shift Pseudomonas sp. acidic lipase Lip I.3 substrate specificity and activity
Chem Commun (Camb)
51 :1330
Panizza P, Cesarini S, Diaz P, Rodriguez Giordano S (2015)
Chem Commun (Camb)
51 :1330