Pazzagli_1994_Eur.J.Pharmacol_254_277

Reference

Title : In vivo regulation of extracellular adenosine levels in the cerebral cortex by NMDA and muscarinic receptors - Pazzagli_1994_Eur.J.Pharmacol_254_277
Author(s) : Pazzagli M , Corsi C , Latini S , Pedata F , Pepeu G
Ref : European Journal of Pharmacology , 254 :277 , 1994
Abstract :

The adenosine concentration in samples of perfusate was determined 24 h after implantation of microdialysis fibre in the cortex. High performance liquid chromatography coupled with a fluorometric detector was used. K+ (100 mM) depolarization was followed by a 2- to 4-fold increase in adenosine efflux. The addition of tetrodotoxin (1 microM) to the perfusate was followed by a decrease in spontaneous and K(+)-evoked adenosine efflux. The increase induced by high K+ was markedly inhibited by the NMDA receptor antagonist, D(-)-2-amino-7-phosphonoheptanoic acid (1 mM, D-AP7), but not by the muscarinic receptor antagonist, atropine (1.5 microM). The acetylcholine esterase inhibitor, physostigmine (7 microM), and the muscarinic receptor agonist, oxotremorine (100 microM), significantly enhanced the K(+)-evoked increase in adenosine. The spontaneous efflux of adenosine was not modified by any of the drugs tested. A neurotoxic lesion of the cholinergic pathway innervating the cortex, although inducing a marked decrease in cortical choline acetyltransferase activity, did not significantly modify the cortical adenosine efflux. It is concluded that, under K(+)-depolarizing conditions, adenosine efflux is triggered by excitatory amino acids and enhanced by muscarinic activation.

PubMedSearch : Pazzagli_1994_Eur.J.Pharmacol_254_277
PubMedID: 8013563

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Citations formats

Pazzagli M, Corsi C, Latini S, Pedata F, Pepeu G (1994)
In vivo regulation of extracellular adenosine levels in the cerebral cortex by NMDA and muscarinic receptors
European Journal of Pharmacology 254 :277

Pazzagli M, Corsi C, Latini S, Pedata F, Pepeu G (1994)
European Journal of Pharmacology 254 :277