Pena-Montes_2024_Biotechnol.Lett__

Reference

Title : ANCUT1, a novel thermoalkaline cutinase from Aspergillus nidulans and its application on hydroxycinnamic acids lipophilization - Pena-Montes_2024_Biotechnol.Lett__
Author(s) : Pena-Montes C , Bermudez-Garcia E , Castro-Ochoa D , Vega-Perez F , Esqueda-Dominguez K , Castro-Rodriguez JA , Gonzalez-Canto A , Segoviano-Reyes L , Navarro-Ocana A , Farres A
Ref : Biotechnol Lett , : , 2024
Abstract :

One of the four cutinases encoded in the Aspergillus nidulans genome, ANCUT1, is described here. Culture conditions were evaluated, and it was found that this enzyme is produced only when cutin is present in the culture medium, unlike the previously described ANCUT2, with which it shares 62% amino acid identity. The differences between them include the fact that ANCUT1 is a smaller enzyme, with experimental molecular weight and pI values of 22 kDa and 6, respectively. It shows maximum activity at pH 9 and 60 degreesC under assayed conditions and retains more than 60% of activity after incubation for 1 h at 60 degreesC in a wide range of pH values (6-10) after incubations of 1 or 3 h. It has a higher activity towards medium-chain esters and can modify long-chain length hydroxylated fatty acids constituting cutin. Its substrate specificity properties allow the lipophilization of alkyl coumarates, valuable antioxidants and its thermoalkaline behavior, which competes favorably with other fungal cutinases, suggests it may be useful in many more applications.

PubMedSearch : Pena-Montes_2024_Biotechnol.Lett__
PubMedID: 38416309
Gene_locus related to this paper: emeni-q5b2c1

Related information

Gene_locus emeni-q5b2c1

Citations formats

Pena-Montes C, Bermudez-Garcia E, Castro-Ochoa D, Vega-Perez F, Esqueda-Dominguez K, Castro-Rodriguez JA, Gonzalez-Canto A, Segoviano-Reyes L, Navarro-Ocana A, Farres A (2024)
ANCUT1, a novel thermoalkaline cutinase from Aspergillus nidulans and its application on hydroxycinnamic acids lipophilization
Biotechnol Lett :

Pena-Montes C, Bermudez-Garcia E, Castro-Ochoa D, Vega-Perez F, Esqueda-Dominguez K, Castro-Rodriguez JA, Gonzalez-Canto A, Segoviano-Reyes L, Navarro-Ocana A, Farres A (2024)
Biotechnol Lett :