Peng_2017_Biomed.Chromatogr_31_

Reference

Title : A novel assay to determine acetylcholinesterase activity: The application potential for screening of drugs against Alzheimer's disease - Peng_2017_Biomed.Chromatogr_31_
Author(s) : Peng L , Rong Z , Wang H , Shao B , Kang L , Qi H , Chen H
Ref : Biomedical Chromatography , 31 : , 2017
Abstract :

Acetycholinesterase (AChE) that regulates hydrolysis of acetylcholine (ACh) in the brain, is an important target for treatment of Alzheimer's disease (AD), a feature of which is ACh deficiency. However, the methods to precisely determine AChE activity are still under development. We developed a new method to exploit acetylcholine-d4 as a surrogate substrate of ACh and measure product choline-d4 via liquid chromatography-tandem mass spectrometry (LC-MS/MS). This assay detected activity of AChE present in the normal mouse brain, which is consistent with the standard Ellman assay that determines products spectrophotometrically. In AD mouse models, the result of LC-MS/MS assay showed significant higher AChE activity than that seen in control normal mice, while treatment of AD mice with an AChE inhibitor, huperzine A, led to partial decreases in AChE activity. Our results suggest that this surrogate-based LC-MS/MS method is a new, sensitive and convenient assay for the determination of AChE activity, providing a useful means for screening active compounds that target AChE.

PubMedSearch : Peng_2017_Biomed.Chromatogr_31_
PubMedID: 28295437

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Citations formats

Peng L, Rong Z, Wang H, Shao B, Kang L, Qi H, Chen H (2017)
A novel assay to determine acetylcholinesterase activity: The application potential for screening of drugs against Alzheimer's disease
Biomedical Chromatography 31 :

Peng L, Rong Z, Wang H, Shao B, Kang L, Qi H, Chen H (2017)
Biomedical Chromatography 31 :