| Title : Quantitative proteomic analysis reveals the mechanism and key esterase of beta-cypermethrin degradation in a bacterial strain from fermented food - Peng_2024_Pestic.Biochem.Physiol_201_105858 |
| Author(s) : Peng C , Tang J , Zhou X , Zhou H , Zhang Y , Wang S , Wang W , Xiang W , Zhang Q , Yu X , Cai T |
| Ref : Pestic Biochem Physiol , 201 :105858 , 2024 |
| Abstract : Beta-cypermethrin (beta-CY) residues in food are an important threat to human health. Microorganisms can degrade beta-CY residues during fermentation of fruits and vegetables, while the mechanism is not clear. In this study, a comprehensively investigate of the degradation mechanism of beta-CY in a food microorganism was conducted based on proteomics analysis. The beta-CY degradation bacteria Gordonia alkanivorans GH-1 was derived from fermented Pixian Doubanjiang. Its crude enzyme extract could degrade 77.11% of beta-CY at a concentration of 45 mg/L within 24 h. Proteomics analysis revealed that the ester bond of beta-CY is broken under the action of esterase to produce 3-phenoxy benzoic acid, which was further degraded by oxidoreductase and aromatic degrading enzyme. The up-regulation expression of oxidoreductase and esterase was confirmed by transcriptome and quantitative reverse transcription PCR. Meanwhile, the expression of esterase Est280 in Escherichia coli BL21 (DE3) resulted in a 48.43% enhancement in the degradation efficiency of beta-CY, which confirmed that this enzyme was the key enzyme in the process of beta-CY degradation. This study reveals the degradation mechanism of beta-CY by microorganisms during food fermentation, providing a theoretical basis for the application of food microorganisms in beta-CY residues. |
| ESTHER : Peng_2024_Pestic.Biochem.Physiol_201_105858 |
| PubMedSearch : Peng_2024_Pestic.Biochem.Physiol_201_105858 |
| PubMedID: 38685237 |
Peng C, Tang J, Zhou X, Zhou H, Zhang Y, Wang S, Wang W, Xiang W, Zhang Q, Yu X, Cai T (2024)
Quantitative proteomic analysis reveals the mechanism and key esterase of beta-cypermethrin degradation in a bacterial strain from fermented food
Pestic Biochem Physiol
201 :105858
Peng C, Tang J, Zhou X, Zhou H, Zhang Y, Wang S, Wang W, Xiang W, Zhang Q, Yu X, Cai T (2024)
Pestic Biochem Physiol
201 :105858