Title : Degradation of complex arabinoxylans by human colonic Bacteroidetes - Pereira_2021_Nat.Commun_12_459 |
Author(s) : Pereira GV , Abdel-Hamid AM , Dutta S , D'Alessandro-Gabazza CN , Wefers D , Farris JA , Bajaj S , Wawrzak Z , Atomi H , Mackie RI , Gabazza EC , Shukla D , Koropatkin NM , Cann I |
Ref : Nat Commun , 12 :459 , 2021 |
Abstract :
Some Bacteroidetes and other human colonic bacteria can degrade arabinoxylans, common polysaccharides found in dietary fiber. Previous work has identified gene clusters (polysaccharide-utilization loci, PULs) for degradation of simple arabinoxylans. However, the degradation of complex arabinoxylans (containing side chains such as ferulic acid, a phenolic compound) is poorly understood. Here, we identify a PUL that encodes multiple esterases for degradation of complex arabinoxylans in Bacteroides species. The PUL is specifically upregulated in the presence of complex arabinoxylans. We characterize some of the esterases biochemically and structurally, and show that they release ferulic acid from complex arabinoxylans. Growth of four different colonic Bacteroidetes members, including Bacteroides intestinalis, on complex arabinoxylans results in accumulation of ferulic acid, a compound known to have antioxidative and immunomodulatory properties. |
PubMedSearch : Pereira_2021_Nat.Commun_12_459 |
PubMedID: 33469030 |
Gene_locus related to this paper: 9bace-k9e1w0 , 9bace-b3c976 , 9bace-e2ncy6 , 9bace-e2ncy7 , 9bace-a0a380yku1 , 9bace-b3c969 , 9bace-b3c974 , 9bace-b3c975 |
Gene_locus | 9bace-k9e1w0 9bace-b3c976 9bace-e2ncy6 9bace-e2ncy7 9bace-a0a380yku1 9bace-b3c969 9bace-b3c974 9bace-b3c975 |
Structure | 6MLY 6NE9 6MOT 6MOU |
Pereira GV, Abdel-Hamid AM, Dutta S, D'Alessandro-Gabazza CN, Wefers D, Farris JA, Bajaj S, Wawrzak Z, Atomi H, Mackie RI, Gabazza EC, Shukla D, Koropatkin NM, Cann I (2021)
Degradation of complex arabinoxylans by human colonic Bacteroidetes
Nat Commun
12 :459
Pereira GV, Abdel-Hamid AM, Dutta S, D'Alessandro-Gabazza CN, Wefers D, Farris JA, Bajaj S, Wawrzak Z, Atomi H, Mackie RI, Gabazza EC, Shukla D, Koropatkin NM, Cann I (2021)
Nat Commun
12 :459