Perez_2002_FEMS.Microbiol.Lett_211_23

Reference

Title : A 45-kDa acetylcholinesterase protoxin of Aeromonas hydrophila: purification and immunogenicity in fish - Perez_2002_FEMS.Microbiol.Lett_211_23
Author(s) : Perez MJ , Rodriguez LA , Fernandez-Briera A , Nieto TP
Ref : FEMS Microbiology Letters , 211 :23 , 2002
Abstract : A rabbit antiserum to the 15-kDa acetylcholinesterase toxin neutralised the lethal effect of the 15-kDa toxin of Aeromonas hydrophila when injected into trout. However, immunisation of fish with the 15-kDa toxoid failed to induce an antibody response, and a higher molecular mass form of this toxin was purified from the extracellular products with the aim of inducing an immune response in fish. The optimal conditions for production of extracellular products by A. hydrophila strain B32 were studied to increase the concentration of this protoxin. The extracellular products were fractionated by molecular exclusion chromatography to yield a purified protoxin with an estimated molecular mass of 45 kDa by SDS-PAGE and which gave a positive reaction in Western blotting with the rabbit anti-15-kDa toxin serum. Since the 45-kDa protoxin showed lower specific acetylcholinesterase activity than the active 15-kDa toxin, the behaviour of the active site was studied using specific inhibitors. This 45-kDa protoxin was 13.3-fold less toxic than the 15-kDa toxin and induced antibody production in fish.
ESTHER : Perez_2002_FEMS.Microbiol.Lett_211_23
PubMedSearch : Perez_2002_FEMS.Microbiol.Lett_211_23
PubMedID: 12052546

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Citations formats

Perez MJ, Rodriguez LA, Fernandez-Briera A, Nieto TP (2002)
A 45-kDa acetylcholinesterase protoxin of Aeromonas hydrophila: purification and immunogenicity in fish
FEMS Microbiology Letters 211 :23

Perez MJ, Rodriguez LA, Fernandez-Briera A, Nieto TP (2002)
FEMS Microbiology Letters 211 :23