Title : Is acetylcholine an autocrine\/paracrine growth factor via the nicotinic alpha7-receptor subtype in the human colon cancer cell line HT-29? - Pettersson_2009_Eur.J.Pharmacol_609_27 |
Author(s) : Pettersson A , Nilsson L , Nylund G , Khorram-Manesh A , Nordgren S , Delbro DS |
Ref : European Journal of Pharmacology , 609 :27 , 2009 |
Abstract :
We used immunochemistry to demonstrate expression of acetylcholine's nicotinic alpha7-receptor subtype in human colon cancer cell line HT-29. Moreover, RT-PCR and immunochemistry showed that choline acetyltransferase and acetylcholine esterase, the enzymes responsible for acetylcholine synthesis and degradation, respectively, localise in HT-29 cells. Bromoacetylcholine bromide, an inhibitor of choline acetyltransferase, significantly attenuated basal cell growth. Our findings suggest that acetylcholine might serve as an autocrine/paracrine-or speculatively, even intracrine-signalling molecule in cell line HT-29, thus contributing to carcinogenesis/cancer progression. |
PubMedSearch : Pettersson_2009_Eur.J.Pharmacol_609_27 |
PubMedID: 19285065 |
Pettersson A, Nilsson L, Nylund G, Khorram-Manesh A, Nordgren S, Delbro DS (2009)
Is acetylcholine an autocrine\/paracrine growth factor via the nicotinic alpha7-receptor subtype in the human colon cancer cell line HT-29?
European Journal of Pharmacology
609 :27
Pettersson A, Nilsson L, Nylund G, Khorram-Manesh A, Nordgren S, Delbro DS (2009)
European Journal of Pharmacology
609 :27