Title : Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp. strain FA8 - Pettinari_2001_Appl.Environ.Microbiol_67_5331 |
Author(s) : Pettinari MJ , Vazquez GJ , Silberschmidt D , Rehm B , Steinbuchel A , Mendez BS |
Ref : Applied Environmental Microbiology , 67 :5331 , 2001 |
Abstract :
Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene (phbC) was found downstream from genes coding for beta-ketothiolase (phbA) and acetoacetyl-coenzyme A reductase (phbB). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Ralstonia eutropha PHB-4 (phbC-negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB. |
PubMedSearch : Pettinari_2001_Appl.Environ.Microbiol_67_5331 |
PubMedID: 11679365 |
Gene_locus related to this paper: azovi-PHBC |
Gene_locus | azovi-PHBC |
Pettinari MJ, Vazquez GJ, Silberschmidt D, Rehm B, Steinbuchel A, Mendez BS (2001)
Poly(3-hydroxybutyrate) synthesis genes in Azotobacter sp. strain FA8
Applied Environmental Microbiology
67 :5331
Pettinari MJ, Vazquez GJ, Silberschmidt D, Rehm B, Steinbuchel A, Mendez BS (2001)
Applied Environmental Microbiology
67 :5331