Title : Novel insights into enzymes inhibitory responses and metabolomic profile of supercritical fluid extract from chestnut shells upon intestinal permeability - Pinto_2024_Food.Res.Int_175_113807 |
Author(s) : Pinto D , Lozano-Castellon J , Margarida Silva A , de la Luz Cadiz-Gurrea M , Segura-Carretero A , Lamuela-Raventos R , Vallverdu-Queralt A , Delerue-Matos C , Rodrigues F |
Ref : Food Res Int , 175 :113807 , 2024 |
Abstract :
The health benefits of chestnut (Castanea sativa) shells (CSs) have been ascribed to phytochemicals, mainly phenolic compounds. Nevertheless, an exhaustive assessment of their intestinal absorption is vital considering a possible nutraceutical application. This study evaluated the bioactivity of CSs extract prepared by Supercritical Fluid Extraction and untargeted metabolomic profile upon in-vitro intestinal permeation across a Caco-2/HT29-MTX co-culture model. The results demonstrated the neuroprotective, hypoglycemic, and hypolipidemic properties of CSs extract by inhibition of acetylcholinesterase, alpha-amylase, and lipase activities. The untargeted metabolic profiling by LC-ESI-LTQ-Orbitrap-MS unveiled almost 60 % of lipids and 30 % of phenolic compounds, with 29 metabolic pathways indicated by enrichment analysis. Among phenolics, mostly phenolic acids, flavonoids, and coumarins permeated the intestinal barrier with most metabolites arising from phase I reactions (reduction, hydrolysis, and hydrogenation) and a minor fraction from phase II reactions (methylation). The permeation rates enhanced in the following order: ellagic acid < o-coumaric acid < p-coumaric acid < ferulaldehyde >= hydroxyferulic acid >= dihydroferulic acid < ferulic acid < trans-caffeic acid < trans-cinnamic acid < dihydrocaffeic acid, with better outcomes for 1000 microg/mL of extract concentration and after 4 h of permeation. Taken together, these findings sustained a considerable in-vitro intestinal absorption of phenolic compounds from CSs extract, enabling them to reach target sites and exert their biological effects. |
PubMedSearch : Pinto_2024_Food.Res.Int_175_113807 |
PubMedID: 38129012 |
Pinto D, Lozano-Castellon J, Margarida Silva A, de la Luz Cadiz-Gurrea M, Segura-Carretero A, Lamuela-Raventos R, Vallverdu-Queralt A, Delerue-Matos C, Rodrigues F (2024)
Novel insights into enzymes inhibitory responses and metabolomic profile of supercritical fluid extract from chestnut shells upon intestinal permeability
Food Res Int
175 :113807
Pinto D, Lozano-Castellon J, Margarida Silva A, de la Luz Cadiz-Gurrea M, Segura-Carretero A, Lamuela-Raventos R, Vallverdu-Queralt A, Delerue-Matos C, Rodrigues F (2024)
Food Res Int
175 :113807