Podar_2007_Appl.Environ.Microbiol_73_3205

Reference

Title : Targeted access to the genomes of low-abundance organisms in complex microbial communities - Podar_2007_Appl.Environ.Microbiol_73_3205
Author(s) : Podar M , Abulencia CB , Walcher M , Hutchison D , Zengler K , Garcia JA , Holland T , Cotton D , Hauser L , Keller M
Ref : Applied Environmental Microbiology , 73 :3205 , 2007
Abstract :

Current metagenomic approaches to the study of complex microbial consortia provide a glimpse into the community metabolism and occasionally allow genomic assemblies for the most abundant organisms. However, little information is gained for the members of the community present at low frequencies, especially those representing yet-uncultured taxa, which include the bulk of the diversity present in most environments. Here we used phylogenetically directed cell separation by fluorescence in situ hybridization and flow cytometry, followed by amplification and sequencing of a fraction of the genomic DNA of several bacterial cells that belong to the TM7 phylum. Partial genomic assembly allowed, for the first time, a look into the evolution and potential metabolism of a soil representative from this group of organisms for which there are no species in stable laboratory cultures. Genomic reconstruction from targeted cells of uncultured organisms isolated directly from the environment represents a powerful approach to access any specific members of a community and an alternative way to assess the community's metabolic potential.

PubMedSearch : Podar_2007_Appl.Environ.Microbiol_73_3205
PubMedID: 17369337
Gene_locus related to this paper: 9bact-a5ks31 , 9bact-a5kt23 , 9bact-a5kt95

Related information

Gene_locus 9bact-a5ks31    9bact-a5kt23    9bact-a5kt95

Citations formats

Podar M, Abulencia CB, Walcher M, Hutchison D, Zengler K, Garcia JA, Holland T, Cotton D, Hauser L, Keller M (2007)
Targeted access to the genomes of low-abundance organisms in complex microbial communities
Applied Environmental Microbiology 73 :3205

Podar M, Abulencia CB, Walcher M, Hutchison D, Zengler K, Garcia JA, Holland T, Cotton D, Hauser L, Keller M (2007)
Applied Environmental Microbiology 73 :3205