Title : Determination of organophosphate and carbamate pesticides in spiked samples of tap water and fruit juices by a biosensor with photothermal detection - Pogacnik_1999_Biosens.Bioelectron_14_569 |
Author(s) : Pogacnik L , Franko M |
Ref : Biosensors & Bioelectronics , 14 :569 , 1999 |
Abstract :
The determination of organophosphate (paraoxon, chlorpyrifos, diazinon) and carbamate (carbaryl, carbofuran) pesticides in spiked drinking water and fruit juices was carried out using a photothermal biosensor. The biosensor consists of a cartridge containing immobilised enzyme acetylcholinesterase (AChE) placed in a flow-injection analysis (FIA) manifold and a photothermal detector based on thermal lens spectrometry. With this approach, 0.2 ng/ml of paraoxon can be detected in less than 15 min. Limits of detection for other organophosphate (chlorpyrifos, diazinon) and carbamate (carbaryl, carbofuran) pesticides varied, depending on their antiacetylcholinesterase (AntiAChE) toxicity, from 1 ng/ml to 4 microg/ml. The biosensor was used for the direct detection of pesticides in spiked tap water and fruit juices without any pretreatment steps. In these cases, the LOD3sigma of 1.5, 2.8 and 4 ng/ml paraoxon in tap water, orange juice and apple juice were obtained, respectively. |
PubMedSearch : Pogacnik_1999_Biosens.Bioelectron_14_569 |
PubMedID: 11459102 |
Pogacnik L, Franko M (1999)
Determination of organophosphate and carbamate pesticides in spiked samples of tap water and fruit juices by a biosensor with photothermal detection
Biosensors & Bioelectronics
14 :569
Pogacnik L, Franko M (1999)
Biosensors & Bioelectronics
14 :569