Pohanka_2011_Toxicol.Mech.Methods_21_585

Reference

Title : Metrifonate alters antioxidant levels and caspase activity in cerebral cortex of Wistar rats - Pohanka_2011_Toxicol.Mech.Methods_21_585
Author(s) : Pohanka M , Novotny L , Pikula J
Ref : Toxicol Mech Methods , 21 :585 , 2011
Abstract : Metrifonate (trichlorfon) is an inhibitor of acetylcholinesterase (AChE). It was used as an Alzheimer's disease (AD) drug; however, the application was withdrawn due to adverse effects. Implication of metrifonate for the antioxidant status and regulation of apoptotic processes was evaluated in the present study. Wistar rats (six per group) were exposed subcutaneously to either 60 or 120 mg/kg of body weight of metrifonate and compared with the controls treated with saline only. Cerebral cortex and liver tissues were collected from animals 40 min after exposure. Activities of AChE, glutathione reductase, glutathione-S-transferase, caspase 3, total protein level, thiobarbituric acid reactive substances, reduced glutathione level and ferric reducing antioxidant power (FRAP) were assayed in the tissue samples. Metrifonate had only lower impact on oxidative stress in the liver. Cerebral cortex tissues had decreased AChE and increased caspase 3 activities as well as the FRAP level. Owing to the novel findings, suitability of metrifonate for AD therapy is discussed.
ESTHER : Pohanka_2011_Toxicol.Mech.Methods_21_585
PubMedSearch : Pohanka_2011_Toxicol.Mech.Methods_21_585
PubMedID: 21943232

Related information

Citations formats

Pohanka M, Novotny L, Pikula J (2011)
Metrifonate alters antioxidant levels and caspase activity in cerebral cortex of Wistar rats
Toxicol Mech Methods 21 :585

Pohanka M, Novotny L, Pikula J (2011)
Toxicol Mech Methods 21 :585

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    [author] => Pohanka M || Novotny L || Pikula J
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    [title] => Metrifonate alters antioxidant levels and caspase activity in cerebral cortex of Wistar rats
    [journal] => Toxicol Mech Methods
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            [content] => Metrifonate (trichlorfon) is an inhibitor of acetylcholinesterase (AChE). It was used as an Alzheimer's disease (AD) drug; however, the application was withdrawn due to adverse effects. Implication of metrifonate for the antioxidant status and regulation of apoptotic processes was evaluated in the present study. Wistar rats (six per group) were exposed subcutaneously to either 60 or 120 mg/kg of body weight of metrifonate and compared with the controls treated with saline only. Cerebral cortex and liver tissues were collected from animals 40 min after exposure. Activities of AChE, glutathione reductase, glutathione-S-transferase, caspase 3, total protein level, thiobarbituric acid reactive substances, reduced glutathione level and ferric reducing antioxidant power (FRAP) were assayed in the tissue samples. Metrifonate had only lower impact on oxidative stress in the liver. Cerebral cortex tissues had decreased AChE and increased caspase 3 activities as well as the FRAP level. Owing to the novel findings, suitability of metrifonate for AD therapy is discussed.
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