Pompili_2003_J.Histochem.Cytochem_51_471

Reference

Title : Biochemical and immunohistochemical evidence for a non-muscle myosin at the neuromuscular junction in bovine skeletal muscle - Pompili_2003_J.Histochem.Cytochem_51_471
Author(s) : Pompili E , De Luca A , Nori SL , Maras B , De Renzis G , Ortolani F , Fumagalli L
Ref : Journal of Histochemistry & Cytochemistry , 51 :471 , 2003
Abstract :

We identified 220-kD protein in bovine skeletal muscle homogenate by affinity chromatography on an agarose column and subsequent SDS-PAGE. Peptide mass fingerprinting (MALDI mass spectrometry) and internal sequence analysis revealed that this protein has homology with several members of the myosin superfamily, particularly with human cardiac beta-myosin heavy chain (beta-MHC). A rabbit polyclonal antibody against the 220-kD protein specifically stained a 220-kD band in Western blots of skeletal muscle homogenate. Immunohistochemical experiments on cryostat sections demonstrated that in skeletal muscle this protein is exclusively localized at the neuromuscular junctions, no immunoreactivity being present at the myofibril level. Because of its relative homology with cardiac beta-MHC, we also investigated the distribution of the 220-kD protein in bovine heart. In cardiac fibers, 220-kD protein-related immunoreactivity was restricted to the intercalated disks, whereas myofibrils were completely devoid of specific immunoreactivity. This distribution pattern was completely different from that of cardiac beta-MHC, which involved myofibrils. Because of the above biochemical and immunohistochemical features, the 220-kD protein we have identified is suggested to be a novel member of the non-muscle (non-sarcomeric) myosin family.

PubMedSearch : Pompili_2003_J.Histochem.Cytochem_51_471
PubMedID: 12642625

Related information

Citations formats

Pompili E, De Luca A, Nori SL, Maras B, De Renzis G, Ortolani F, Fumagalli L (2003)
Biochemical and immunohistochemical evidence for a non-muscle myosin at the neuromuscular junction in bovine skeletal muscle
Journal of Histochemistry & Cytochemistry 51 :471

Pompili E, De Luca A, Nori SL, Maras B, De Renzis G, Ortolani F, Fumagalli L (2003)
Journal of Histochemistry & Cytochemistry 51 :471