Portela_2019_Neurochem.Res_44_2202

Reference

Title : Ilex paraguariensis Attenuates Changes in Mortality, Behavioral and Biochemical Parameters Associated to Methyl Malonate or Malonate Exposure in Drosophila melanogaster - Portela_2019_Neurochem.Res_44_2202
Author(s) : Portela JL , Bianchini MC , Boligon AA , Carrico MRS , Roehrs R , Soares FAA , de Gomes MG , Hassan W , Puntel RL
Ref : Neurochem Res , 44 :2202 , 2019
Abstract :

Methylmalonic acidemia is a genetic disease characterized by accumulation of organic acids, such as methylmalonic (MMA) and malonic (MA) acids. Considering that the accumulation of MMA and MA causes several damages due to oxidative stress, antioxidants are thought to play a pivotal role in preventing deleterious effects associated with exposure to such compounds. Ilex paraguariensis (IP) was used here to test the hypothesis that supplementation with the aqueous extract of this plant could exert protective effect against MMA or MA induced mortality, behavioral and/or biochemical changes in Drosophila melanogaster (DM). Initially, a curve time- and dose-response to MMA (1-10 mM), MA (1-10 mM) and IP (63-500 muM) was performed. Thereafter, flies were concomitantly exposed to MA (5 mM), MMA (5 mM) and/or IP (250 mug/mL) during 15 days for survival assay, and for 48 hs to MA (1 or 5 mM), MMA (1 or 5 mM) and/or IP (250 mug/mL) for subsequent investigations. Both MMA and MA exposure resulted in higher incidence of mortality, a worse performance in the negative geotaxis assay and increased locomotion in open-field test as compared with control group. Furthermore, a marked increase in non-protein thiol (NPSH) and in thiobarbituric acid reactive substances (TBARS) levels, decrease in superoxide dismutase (SOD), catalase and acetylcholinesterase (AChE) activities, and decrease in MTT and resazurin reduction were noted in MMA or MA treated groups. IP treatment offered significant protection against all alterations associated to MMA or MA exposure. This study confirm the hypothesis that supplementation with IP offers protection against changes associated to MMA or MA exposure in DM, due, at least in part, to its antioxidant effect.

PubMedSearch : Portela_2019_Neurochem.Res_44_2202
PubMedID: 31422521

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Citations formats

Portela JL, Bianchini MC, Boligon AA, Carrico MRS, Roehrs R, Soares FAA, de Gomes MG, Hassan W, Puntel RL (2019)
Ilex paraguariensis Attenuates Changes in Mortality, Behavioral and Biochemical Parameters Associated to Methyl Malonate or Malonate Exposure in Drosophila melanogaster
Neurochem Res 44 :2202

Portela JL, Bianchini MC, Boligon AA, Carrico MRS, Roehrs R, Soares FAA, de Gomes MG, Hassan W, Puntel RL (2019)
Neurochem Res 44 :2202