Prado-Ochoa_2014_Vet.Parasitol_199_215

Reference

Title : The action of two ethyl carbamates on acetylcholinesterase and reproductive organs of Rhipicephalus microplus - Prado-Ochoa_2014_Vet.Parasitol_199_215
Author(s) : Prado-Ochoa MG , Ramirez-Noguera P , Diaz-Torres R , Garrido-Farina GI , Vazquez-Valadez VH , Velazquez-Sanchez AM , Munoz-Guzman MA , Angeles E , Alba-Hurtado F
Ref : Vet Parasitol , 199 :215 , 2014
Abstract :

The effects produced by the new synthetic carbamates ethyl-(4-bromophenyl) carbamate and ethyl-(4-chlorophenyl) carbamate on the acetylcholinesterase (AChE) activity, egg structure and reproductive organs of two Rhipicephalus microplus strains were evaluated. Inhibition kinetic parameters showed that the studied carbamates are weak inhibitors and have a low affinity for R. microplus AChE. Histologically, in oocytes from carbamate-treated engorged female ticks, a loss of shape, cytoplasmic vacuoles, decreased chorion deposition, alterations in cytoplasmic granularity and irregular membranes were observed. In oocyte germinal vesicles, a loss of shape, nucleolar fragmentation and membrane alterations with degenerative signs were observed. The ovarian epithelium was vacuolated, flattened, eroded and contained pyknotic nuclei. These alterations were observed from the first day and persisted and increased in severity until day 7 post-treatment. The ovaries from carbamate-treated ticks had fewer stage IV-V oocytes and more stage I-II oocytes. Additionally, eggs produced by the treated ticks had a modified appearance, decreased size, a reduced superficial waxy layer and a loss of viability. The results of this study show that the effects of carbamates on R. microplus were independent of AChE inhibition and show that the morphological alterations in the reproductive organs were due to carbamate actions on the vitellogenesis and viability of the ovarian cells.

PubMedSearch : Prado-Ochoa_2014_Vet.Parasitol_199_215
PubMedID: 24315691

Related information

Citations formats

Prado-Ochoa MG, Ramirez-Noguera P, Diaz-Torres R, Garrido-Farina GI, Vazquez-Valadez VH, Velazquez-Sanchez AM, Munoz-Guzman MA, Angeles E, Alba-Hurtado F (2014)
The action of two ethyl carbamates on acetylcholinesterase and reproductive organs of Rhipicephalus microplus
Vet Parasitol 199 :215

Prado-Ochoa MG, Ramirez-Noguera P, Diaz-Torres R, Garrido-Farina GI, Vazquez-Valadez VH, Velazquez-Sanchez AM, Munoz-Guzman MA, Angeles E, Alba-Hurtado F (2014)
Vet Parasitol 199 :215