Title : Analysing organophosphorus pesticides in wines using graphitized carbon black extraction cartridges - Prieto_1999_Food.Addit.Contam_16_57 |
Author(s) : Prieto A , Ettiene G , Medina D , Buscema I , Gonzalez G , Araujo L |
Ref : Food Additives & Contaminants , 16 :57 , 1999 |
Abstract :
A quick and simple method was developed to determine the residues of 15 organophosphorus pesticides of different polarity in grape wine, based on liquid-solid extraction with graphitized carbon black cartridges and capillary gas chromatography. Aliquots of diluted wine (10 ml wine + 40 ml deionized water) and 250 mg graphitized carbon black cartridges were used to extract the analytes. The pesticides were eluted with 2 x 0.5 ml acetone and 3 x 2.5 ml ethyl acetate/acetone (80:20). The eluates were then dried with anhydrous sodium sulphate and concentrated to 1 ml under a nitrogen current. After adding triphenylphosphate internal standard (i.s.), this extract was analysed by capillary gas chromatography using a nitrogen-phosphorus detector (GC-NPD). Pesticide recovery percentages at levels of 0.01 and 0.02 microgram/ml were between 74% and 111%, with a 2-13% relative standard deviation interval. The chromatograms of extracts had few co-extracted species peaks, with no analytical interference. Dilution of the grape wine samples removed most of the pigments, thus producing clear extracts. The results indicate that liquid-solid extraction from wine samples using graphitized carbon is fast (25 min per sample), efficient and reproducible for analysing organophosphorus pesticides of different polarity. |
PubMedSearch : Prieto_1999_Food.Addit.Contam_16_57 |
PubMedID: 10435073 |
Prieto A, Ettiene G, Medina D, Buscema I, Gonzalez G, Araujo L (1999)
Analysing organophosphorus pesticides in wines using graphitized carbon black extraction cartridges
Food Additives & Contaminants
16 :57
Prieto A, Ettiene G, Medina D, Buscema I, Gonzalez G, Araujo L (1999)
Food Additives & Contaminants
16 :57