Prior_1993_J.Pharmacol.Toxicol.Methods_30_1

Reference

Title : Electrophysiological analysis of transmission at the skeletal neuromuscular junction - Prior_1993_J.Pharmacol.Toxicol.Methods_30_1
Author(s) : Prior C , Dempster J , Marshall IG
Ref : Journal of Pharmacological & Toxicological Methods , 30 :1 , 1993
Abstract :

The review is divided into two sections. The first deals with methods and problems associated with performing electrophysiological experimentation on the skeletal muscle neuromuscular junction. The second section concentrates on the computer analysis of electrophysiological data. In the first section, the various techniques available for producing skeletal muscle paralysis are described. These include the use of pharmacological manipulations, such as an excess of magnesium ions or a competitive postjunctional nicotinic acetylcholine antagonist, physiological manipulations, such as cutting the muscle fibers, and the muscle fiber sodium channel toxin, mu-conotoxin. Also, in this section, a comparison is made of the use of voltage- and current-recording techniques, including descriptions of, and solutions to, the problems associated with membrane capacitance, nonlinear summation, membrane space constant, and electrical and mechanical interference. In the second section, details are given of the types of computer system commonly used for the analysis of electrophysiological data and also the requirements of the data analysis software. The use of computer algorithms for signal detection, signal evaluation, signal averaging, and curve fitting are qualitatively described, along with some of the problems and pitfalls often associated with these methods.

PubMedSearch : Prior_1993_J.Pharmacol.Toxicol.Methods_30_1
PubMedID: 8241541

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Citations formats

Prior C, Dempster J, Marshall IG (1993)
Electrophysiological analysis of transmission at the skeletal neuromuscular junction
Journal of Pharmacological & Toxicological Methods 30 :1

Prior C, Dempster J, Marshall IG (1993)
Journal of Pharmacological & Toxicological Methods 30 :1