Qian_2025_Food.Chem_493_146126

In order to improve the hydrolysis performance of cross-linked enzyme aggregates and the defects of the smaller particles affecting the reuse, the lipase added soybean protein, silica nanoparticles as adsorption carrier preparation lipase cross-linked aggregates were studied. The prepared cross-linked enzyme aggregates showed lipase activity recovery of 94.34 +/- 0.81 %, and particle size reached about 1100 nm, which is favorable for reuse. The thermal stability is significantly improved, the half-life of lipase activity at 65 degreesC is 5.2 times of free lipase. The lipase cross-linked aggregates were applied to hydrolyze soybean phospholipids in two-phase system consisting of n-hexane and water for 8 h, Hydrolysis rate of 125.1 +/- 1.2 % and hydrolysis speed of 2.57 +/- 0.05 g/h, which was 3.5 times of free lipase. This study provides a technical basis for new stratagem of preparation cross-linked enzyme aggregates with silica nanoparticles as carrier and soybean protein as cross-linking additive, and application of lipase for hydrolysis of soybean phospholipids.