Rabesandratana_1998_Blood_91_2573

Reference

Title : Decay-accelerating factor (CD55) and membrane inhibitor of reactive lysis (CD59) are released within exosomes during In vitro maturation of reticulocytes - Rabesandratana_1998_Blood_91_2573
Author(s) : Rabesandratana H , Toutant JP , Reggio H , Vidal M
Ref : Blood , 91 :2573 , 1998
Abstract :

Exosomes are membrane vesicles released by reticulocytes during their maturation into erythrocytes. They have a clearing function because of their enrichment with some proteins known to decrease or disappear from the cell surface during maturation, eg, acetylcholinesterase (AChE) and transferrin receptor (TfR), respectively. To better understand the molecular events leading to protein sorting in exosomes, we analyzed the expression of glycosylphosphatidylinositol (GPI)-anchored proteins on the exosome surface through a technique involving bead coupling and flow cytometry immunodetection. The presence of AChE, decay-accelerating factor (DAF), membrane inhibitor of reactive lysis (MIRL), and lymphocyte function-associated antigen 3 (LFA-3) on the surface of exosomes obtained from normal and paroxysmal nocturnal hemoglobinuria (PNH) reticulocytes, suggests that (1) the GPI anchor is efficiently sorted during exosome formation, (2) exosome release could account for the observed discrepancy in GPI-protein expression between reticulocytes and erythrocytes from PNH patients, and (3) exosomes could have another physiologic function related to controlling membrane attack complex formation.

PubMedSearch : Rabesandratana_1998_Blood_91_2573
PubMedID: 9516159

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Citations formats

Rabesandratana H, Toutant JP, Reggio H, Vidal M (1998)
Decay-accelerating factor (CD55) and membrane inhibitor of reactive lysis (CD59) are released within exosomes during In vitro maturation of reticulocytes
Blood 91 :2573

Rabesandratana H, Toutant JP, Reggio H, Vidal M (1998)
Blood 91 :2573