Rachid_2006_Chem.Biol_13_667

Reference

Title : Molecular and biochemical studies of chondramide formation-highly cytotoxic natural products from Chondromyces crocatus Cm c5 - Rachid_2006_Chem.Biol_13_667
Author(s) : Rachid S , Krug D , Kunze B , Kochems I , Scharfe M , Zabriskie TM , Blocker H , Muller R
Ref : Chemical Biology , 13 :667 , 2006
Abstract :

The jaspamide/chondramide family of depsipeptides are mixed PKS/NRPS natural products isolated from marine sponges and a terrestrial myxobacterium that potently affect the function of the actin cytoskeleton. As a first step to improve production in heterologous host cells and permit genetic approaches to novel analogs, we have cloned and characterized the chondramide biosynthetic genes from the myxobacterium Chondromyces crocatus Cm c5. In addition to the expected PKS and NRPS genes, the cluster encodes a rare tyrosine aminomutase for beta-tyrosine formation and a previously unknown tryptophan-2-halogenase. Conditions for gene transfer into C. crocatus Cm c5 were developed, and inactivation of several genes corroborated their proposed function and served to define the boundaries of the cluster. Biochemical characterization of the final NRPS adenylation domain confirmed the direct activation of beta-tyrosine, and fluorinated chondramides were produced through precursor-directed biosynthesis.

PubMedSearch : Rachid_2006_Chem.Biol_13_667
PubMedID: 16793524
Gene_locus related to this paper: choco-chsad

Related information

Gene_locus choco-chsad

Citations formats

Rachid S, Krug D, Kunze B, Kochems I, Scharfe M, Zabriskie TM, Blocker H, Muller R (2006)
Molecular and biochemical studies of chondramide formation-highly cytotoxic natural products from Chondromyces crocatus Cm c5
Chemical Biology 13 :667

Rachid S, Krug D, Kunze B, Kochems I, Scharfe M, Zabriskie TM, Blocker H, Muller R (2006)
Chemical Biology 13 :667