Raj_2015_Methods.Mol.Biol_1282_165

Reference

Title : Identification of protein receptors for coronaviruses by mass spectrometry - Raj_2015_Methods.Mol.Biol_1282_165
Author(s) : Raj VS , Lamers MM , Smits SL , Demmers JA , Mou H , Bosch BJ , Haagmans BL
Ref : Methods Mol Biol , 1282 :165 , 2015
Abstract :

As obligate intracellular parasites, viruses need to cross the plasma membrane and deliver their genome inside the cell. This step is initiated by the recognition of receptors present on the host cell surface. Receptors can be major determinants of tropism, host range, and pathogenesis. Identifying virus receptors can give clues to these aspects and can lead to the design of intervention strategies. Interfering with receptor recognition is an attractive antiviral therapy, since it occurs before the viral genome has reached the relative safe haven within the cell. This chapter describes the use of an immunoprecipitation approach with Fc-tagged viral spike proteins followed by mass spectrometry to identify and characterize the receptor for the Middle East respiratory syndrome coronavirus. This technique can be adapted to identify other viral receptors.

PubMedSearch : Raj_2015_Methods.Mol.Biol_1282_165
PubMedID: 25720480

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Citations formats

Raj VS, Lamers MM, Smits SL, Demmers JA, Mou H, Bosch BJ, Haagmans BL (2015)
Identification of protein receptors for coronaviruses by mass spectrometry
Methods Mol Biol 1282 :165

Raj VS, Lamers MM, Smits SL, Demmers JA, Mou H, Bosch BJ, Haagmans BL (2015)
Methods Mol Biol 1282 :165