Ramallo_2015_J.Sep.Sci_38_3788

Reference

Title : A reversed-phase compatible thin-layer chromatography autography for the detection of acetylcholinesterase inhibitors - Ramallo_2015_J.Sep.Sci_38_3788
Author(s) : Ramallo IA , Garcia P , Furlan RL
Ref : J Sep Sci , 38 :3788 , 2015
Abstract :

A dual readout autographic assay to detect acetylcholinesterase inhibitors present in complex matrices adsorbed on reversed-phase or normal-phase thin-layer chromatography plates is described. Enzyme gel entrapment with an amphiphilic copolymer was used for assay development. The effects of substrate and enzyme concentrations, pH, incubation time, and incubation temperature on the sensitivity and the detection limit of the assay were evaluated. Experimental design and response surface methodology were used to optimize conditions with a minimum number of experiments. The assay allowed the detection of 0.01% w/w of physostigmine in both a spiked Sonchus oleraceus L. extract chromatographed on normal phase and a spiked Pimenta racemosa (Mill.) J.W. Moore leaf essential oil chromatographed on reversed phase. Finally, the reversed-phase thin-layer chromatography assay was applied to reveal the presence of an inhibitor in the Cymbopogon citratus (DC.) Stapf essential oil. The developed assay is able to detect acetylcholinesterase inhibitors present in complex matrixes that were chromatographed in normal phase or reversed-phase thin-layer chromatography. The detection limit for physostigmine on both normal and reversed phase was of 1x10(-4) mug. The results can be read by a change in color and/or a change in fluorescence.

PubMedSearch : Ramallo_2015_J.Sep.Sci_38_3788
PubMedID: 26489065

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Citations formats

Ramallo IA, Garcia P, Furlan RL (2015)
A reversed-phase compatible thin-layer chromatography autography for the detection of acetylcholinesterase inhibitors
J Sep Sci 38 :3788

Ramallo IA, Garcia P, Furlan RL (2015)
J Sep Sci 38 :3788