| Title : Production of a novel extracellular acidic lipase from Pseudomonas gessardii using slaughterhouse waste as a substrate - Ramani_2010_J.Ind.Microbiol.Biotechnol_37_531 |
| Author(s) : Ramani K , Chockalingam E , Sekaran G |
| Ref : J Ind Microbiol Biotechnol , 37 :531 , 2010 |
| Abstract : An isolate exhibiting high extracellular lipolytic activity was identified as Pseudomonas gessardii by 16S rDNA gene sequence analysis. The slaughterhouse waste, goat tallow, was used as a lipid substrate for the production of acidic lipase by P. gessardii. The maximum lipase activity of 156 U/ml was observed at an acidic pH of 3.5 and at 0.31 g substrate concentration. The purification steps resulted in the isolation of acidic lipase with a specific activity of 1,473 U/mg and a molecular weight of 94 kDa. One interesting feature of this purified lipase is its stability at highly acidic pH ranging from 2.0 to 5.5 with a high molecular weight. The amino acid composition was determined using HPLC. This acidic lipase has potential applications in the medicinal field as a substitute for pancreatic lipases for enzyme therapy, oleochemical and in biotechnological industries. |
| ESTHER : Ramani_2010_J.Ind.Microbiol.Biotechnol_37_531 |
| PubMedSearch : Ramani_2010_J.Ind.Microbiol.Biotechnol_37_531 |
| PubMedID: 20204455 |
Ramani K, Chockalingam E, Sekaran G (2010)
Production of a novel extracellular acidic lipase from Pseudomonas gessardii using slaughterhouse waste as a substrate
J Ind Microbiol Biotechnol
37 :531
Ramani K, Chockalingam E, Sekaran G (2010)
J Ind Microbiol Biotechnol
37 :531