Ramirez-Romero_1997_Microbiology_143 ( Pt 8)_2825

Reference

Title : Sequence, localization and characteristics of the replicator region of the symbiotic plasmid of Rhizobium etli - Ramirez-Romero_1997_Microbiology_143 ( Pt 8)_2825
Author(s) : Ramirez-Romero MA , Bustos P , Girard L , Rodriguez O , Cevallos MA , Davila G
Ref : Microbiology , 143 ( Pt 8) :2825 , 1997
Abstract :

The replicator region of the symbiotic plasmid of Rhizobium etli CFN42 was cloned and sequenced. A plasmid derivative (pH3) harbouring a 5-6 kb HindIII fragment from the symbiotic plasmid was found to be capable of independent replication and eliminated the symbiotic plasmid when introduced into a R. etli CFNX101 strain (a recA derivative). The stability and the copy number of pH3 were the same as that of the symbiotic plasmid, indicating that the information required for stable replication and incompatibility resides in the 5.6 kb HindIII fragment. The sequence analysis of this fragment showed the presence of three ORFs similar in sequence analysis of this fragment showed the presence of three ORFs similar in sequence and organization to repA, repB and repC described for the replicator regions of the Agrobacterium plasmids pTiB653 and pRiA4b and for the R. leguminosarum cryptic plasmid pRL8JI. Hybridization studies showed that p42d-like replicator sequences are found in the symbiotic plasmids of other R. etli strains and in a 'cryptic' plasmid of R. tropici.

PubMedSearch : Ramirez-Romero_1997_Microbiology_143 ( Pt 8)_2825
PubMedID: 9274036
Gene_locus related to this paper: rhiet-YP051 , rhiet-YP059 , rhiet-YP061

Related information

Gene_locus rhiet-YP051    rhiet-YP059    rhiet-YP061

Citations formats

Ramirez-Romero MA, Bustos P, Girard L, Rodriguez O, Cevallos MA, Davila G (1997)
Sequence, localization and characteristics of the replicator region of the symbiotic plasmid of Rhizobium etli
Microbiology 143 ( Pt 8) :2825

Ramirez-Romero MA, Bustos P, Girard L, Rodriguez O, Cevallos MA, Davila G (1997)
Microbiology 143 ( Pt 8) :2825