| Title : Crystallization and preliminary X-ray analysis of a lipase from Staphylococcus hyicus - Ransac_1995_J.Struct.Biol_114_153 |
| Author(s) : Ransac S , Blaauw M , Dijkstra BW , Slotboom AT , Boots JW , Verheij HM |
| Ref : J Struct Biol , 114 :153 , 1995 |
|
Abstract :
Single cyrstals of a lipase from Staphylococcus hyicus have been obtained using a combination of 18 to 24% dimethylsulfoxide and 10% isopropanol as a precipitant. The crystals grow at 4 degrees C in 2-3 months. They belong to the orthorhombic space group P212121 with a = 73.31 A, b = 77.96 A, and c = 169.81 A, with two protein molecules per asymmetrical unit. The crystals diffract to at least 2.8 A resolution and are suitable for an X-ray structure analysis. |
| PubMedSearch : Ransac_1995_J.Struct.Biol_114_153 |
| PubMedID: 7612398 |
Ransac S, Blaauw M, Dijkstra BW, Slotboom AT, Boots JW, Verheij HM (1995)
Crystallization and preliminary X-ray analysis of a lipase from Staphylococcus hyicus
J Struct Biol
114 :153
Ransac S, Blaauw M, Dijkstra BW, Slotboom AT, Boots JW, Verheij HM (1995)
J Struct Biol
114 :153