Raseta_2025_Nat.Prod.Res__1

Fomitopsis betulina (birch polypore) is a medicinal polypore with limited biochemical characterisation. This study provides the first evidence of polyamines and a detailed phenolic profile in the 80% methanolic (MeOH) extract of its fruiting bodies. HPLC analysis revealed spermidine (60.21 +/- 0.97 mg/kg d.w.), spermine (41.71 +/- 2.56 mg/kg d.w.), and putrescine (0.97 +/- 0.10 mg/kg d.w.), with spermidine as the predominant form. Gallic acid (14,415.07 +/- 1,297.36 ng/g d.e.) and quinic acid (11,332.40 +/- 1,133.24 ng/g d.e.) were accompanied by apiin (514.81 +/- 25.74 ng/g d.e.), reported here for the first time in this species. The MeOH extract exhibited notable antioxidant capacity (DPPH: 2.99 +/- 0.11 mM TE/g; ABTS: 63.50 +/- 0.51 mM TE/g) and strong acetylcholinesterase inhibition (99.03 +/- 5.52%). These findings highlight F. betulina as a source of polyamines and phenolics with promising antioxidant and neuroprotective potential.