Title : Phytochemical analysis and anticholinesterase activity of aril of Myristica fragrans Houtt - Rastegari_2022_BMC.Chem_16_106 |
Author(s) : Rastegari A , Manayi A , Rezakazemi M , Eftekhari M , Khanavi M , Akbarzadeh T , Saeedi M |
Ref : BMC Chem , 16 :106 , 2022 |
Abstract :
In this study, the ethyl acetate fraction of Myristica fragrans Houtt. was investigated for its in vitro anticholinesterase activity as well as neuroprotectivity against H(2)O(2)-induced cell death in PC12 neuronal cells and the ability to chelate bio-metals (Zn(2+), Fe(2+), and Cu(2+)). The fraction was inactive toward acetylcholinesterase (AChE); however, it inhibited the butyrylcholinesterase (BChE) with IC(50) value of 68.16 microg/mL, compared with donepezil as the reference drug (IC(50) = 1.97 microg/mL) via Ellman's method. It also showed good percentage of neuroprotection (86.28% at 100 microg/mL) against H(2)O(2)-induced neurotoxicity and moderate metal chelating ability toward Zn(2+), Fe(2+), and Cu(2+). The phytochemical study led to isolation and identification of malabaricone A (1), malabaricone C (2), 4-(4-(3,4-dimethoxyphenyl)-2,3-dimethylbutyl)benzene-1,2-diol (3), nectandrin B (4), macelignan (5), and 4-(4-(benzo[d][1,3]dioxol-5-yl)-1-methoxy-2,3-dimethylbutyl)-2-methoxyphenol (6) which were assayed for their cholinesterase (ChE) inhibitory activity. Compounds 1 and 3 were not previously reported for M. fragrans. Among isolated compounds, compound 2 showed the best activity toward both AChE and BChE with IC(50) values of 25.02 and 22.36smicroM, respectively, compared with donepezil (0.07 and 4.73smicroM, respectively). |
PubMedSearch : Rastegari_2022_BMC.Chem_16_106 |
PubMedID: 36437466 |
Rastegari A, Manayi A, Rezakazemi M, Eftekhari M, Khanavi M, Akbarzadeh T, Saeedi M (2022)
Phytochemical analysis and anticholinesterase activity of aril of Myristica fragrans Houtt
BMC Chem
16 :106
Rastegari A, Manayi A, Rezakazemi M, Eftekhari M, Khanavi M, Akbarzadeh T, Saeedi M (2022)
BMC Chem
16 :106