Ren_2002_Arch.Insect.Biochem.Physiol_51_103

Reference

Title : Mechanisms of monocrotophos resistance in cotton bollworm, Helicoverpa armigera (Hubner) - Ren_2002_Arch.Insect.Biochem.Physiol_51_103
Author(s) : Ren X , Han Z , Wang Y
Ref : Archives of Insect Biochemistry & Physiology , 51 :103 , 2002
Abstract :

Insensitive acetylcholinesterase was identified as a resistance mechanism by comparing biochemical analysis with a laboratory selected monocrotophos resistant cotton bollworm (RR: 200) and the susceptible strain. The cDNA encoding AChE was cloned by the method of RACE (rapid amplification of cDNA ends). The complete AChE gene deduced from the cDNA consisted of a putative signal peptide of 32 amino acid residues, a mature protein of 615 residues, 5' untranslated regions (UTR) of 315 bp and 3' UTR of 324 bp. The coding sequence had a high degree of homology to the AChE from other insect species reported in the GenBank. After comparing analysis of the entire AChE gene sequence from 5 resistant and 6 susceptible cotton bollworm individuals, nine mutations were identified. One of them, the Ala/Thr mutation, is likely to be responsible for the AChE insensitivity to monocrotophos.

PubMedSearch : Ren_2002_Arch.Insect.Biochem.Physiol_51_103
PubMedID: 12386838
Gene_locus related to this paper: helam-ACHE

Related information

Mutation A585T_helam-ACHE    R4Q_helam-ACHE
Gene_locus helam-ACHE

Citations formats

Ren X, Han Z, Wang Y (2002)
Mechanisms of monocrotophos resistance in cotton bollworm, Helicoverpa armigera (Hubner)
Archives of Insect Biochemistry & Physiology 51 :103

Ren X, Han Z, Wang Y (2002)
Archives of Insect Biochemistry & Physiology 51 :103