| Title : Cloning and Protein Expression of CpEst in Komagataella phaffii - Reyes-Reyes_2027_Methods.Mol.Biol_3064_75 |
| Author(s) : Reyes-Reyes AL , Torres-Anorve DA , Valero F , Sandoval G |
| Ref : Methods Mol Biol , 3064 :75 , 2027 |
|
Abstract :
Esterases are a class of enzymes that catalyze the hydrolysis of ester bonds in various substrates. This chapter presents a methodology for the heterologous expression of Carica papaya esterase (CpEST) in the yeast Komagataella phaffii. The aim is to provide a detailed protocol for the efficient production and characterization of CpEST, highlighting the advantages of using a yeast-based system for scalable production and extracellular secretion. This approach enhances the potential for utilizing plant-derived esterases and lipases in industrial applications. |
| PubMedSearch : Reyes-Reyes_2027_Methods.Mol.Biol_3064_75 |
| PubMedID: 42778849 |
Reyes-Reyes AL, Torres-Anorve DA, Valero F, Sandoval G (2027)
Cloning and Protein Expression of CpEst in Komagataella phaffii
Methods Mol Biol
3064 :75
Reyes-Reyes AL, Torres-Anorve DA, Valero F, Sandoval G (2027)
Methods Mol Biol
3064 :75