Ritpitakphong_2016_New.Phytol_210_1033

Reference

Title : The microbiome of the leaf surface of Arabidopsis protects against a fungal pathogen - Ritpitakphong_2016_New.Phytol_210_1033
Author(s) : Ritpitakphong U , Falquet L , Vimoltust A , Berger A , Metraux JP , L'Haridon F
Ref : New Phytol , 210 :1033 , 2016
Abstract :

We have explored the importance of the phyllosphere microbiome in plant resistance in the cuticle mutants bdg (BODYGUARD) or lacs2.3 (LONG CHAIN FATTY ACID SYNTHASE 2) that are strongly resistant to the fungal pathogen Botrytis cinerea. The study includes infection of plants under sterile conditions, 16S ribosomal DNA sequencing of the phyllosphere microbiome, and isolation and high coverage sequencing of bacteria from the phyllosphere. When inoculated under sterile conditions bdg became as susceptible as wild-type (WT) plants whereas lacs2.3 mutants retained the resistance. Adding washes of its phyllosphere microbiome could restore the resistance of bdg mutants, whereas the resistance of lacs2.3 results from endogenous mechanisms. The phyllosphere microbiome showed distinct populations in WT plants compared to cuticle mutants. One species identified as Pseudomonas sp isolated from the microbiome of bdg provided resistance to B. cinerea on Arabidopsis thaliana as well as on apple fruits. No direct activity was observed against B. cinerea and the action of the bacterium required the plant. Thus, microbes present on the plant surface contribute to the resistance to B. cinerea. These results open new perspectives on the function of the leaf microbiome in the protection of plants.

PubMedSearch : Ritpitakphong_2016_New.Phytol_210_1033
PubMedID: 26725246

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Citations formats

Ritpitakphong U, Falquet L, Vimoltust A, Berger A, Metraux JP, L'Haridon F (2016)
The microbiome of the leaf surface of Arabidopsis protects against a fungal pathogen
New Phytol 210 :1033

Ritpitakphong U, Falquet L, Vimoltust A, Berger A, Metraux JP, L'Haridon F (2016)
New Phytol 210 :1033