Rodrigues_2019_Redox.Rep_24_34

OBJECTIVE: Investigate Vitamin D3 (VD3) effect on the Acetylcholinesterase (AChE), oxidative damage and behavioral tests in animals subjected to Intracerebroventicular injection of Streptozotocin (ICV-STZ) simulating a Sporadic Dementia of Alzheimer's Type (SDAT) and treated with VD3 (21 days). METHODS: Animals were divided into eight groups: Vehicle, VD12.5 mug/kg, VD42 mug/kg, VD125 mug/kg, STZ, STZ+VD12.5 mug/kg, STZ+VD42 mug/kg, STZ+VD125 mug/kg. RESULTS: VD3 prevented the increase in AChE in groups of VD42 microg/kg and VD125 microg/kg; in AChE of synaptossomes and TBARS levels prevented the increase in group VD125 microg/kg; in ROS levels there was not a significant difference; for the Carbonyl Content all doses prevented the increase. Total Thiols prevent the decrease in VD42 microg/kg and VD125 microg/kg, and Reduced Glutathione prevented the decrease in VD125 microg/kg, Oxidized Glutathione prevented the increase in VD125 microg/kg. In relation to behavioral tests, the VD3 prevented the increase in time to find (days 2 and 3), in the time to find the platform (day 3) and in time spent in the quadrant (day 2). However, in relation to crossings there was not difference in groups. These results indicated the therapeutic effect of the VD3 in model of STZ in rats.