| Title : A Novel Thermo-Alkaline Stable GDSL\/SGNH Esterase with Broad Substrate Specificity from a Deep-Sea Pseudomonas sp - Rodriguez-Mejia_2024_Mar.Biotechnol.(NY)__ |
| Author(s) : Rodriguez-Mejia JL , Hidalgo-Manzano IA , Muriel-Millan LF , Rivera-Gomez N , Sahonero-Canavesi DX , Castillo E , Pardo-Lopez L |
| Ref : Mar Biotechnol (NY) , : , 2024 |
| Abstract : Marine environments harbor a plethora of microorganisms that represent a valuable source of new biomolecules of biotechnological interest. In particular, enzymes from marine bacteria exhibit unique properties due to their high catalytic activity under various stressful and fluctuating conditions, such as temperature, pH, and salinity, fluctuations which are common during several industrial processes. In this study, we report a new esterase (EstGoM) from a marine Pseudomonas sp. isolated at a depth of 1000 m in the Gulf of Mexico. Bioinformatic analyses revealed that EstGoM is an autotransporter esterase (type Va) and belongs to the lipolytic family II, forming a new subgroup. The purified recombinant EstGoM, with a molecular mass of 67.4 kDa, showed the highest hydrolytic activity with p-nitrophenyl octanoate (p-NP C8), although it was also active against p-NP C4, C5, C10, and C12. The optimum pH and temperature for EstGoM were 9 and 60 degreesC, respectively, but it retained more than 50% of its activity over the pH range of 7-11 and temperature range of 10-75 degreesC. In addition, EstGoM was tolerant of up to 1 M NaCl and resistant to the presence of several metal ions, detergents, and chemical reagents, such as EDTA and beta-mercaptoethanol. The enzymatic properties of EstGoM make it a potential candidate for several industrial applications. |
| ESTHER : Rodriguez-Mejia_2024_Mar.Biotechnol.(NY)__ |
| PubMedSearch : Rodriguez-Mejia_2024_Mar.Biotechnol.(NY)__ |
| PubMedID: 38691271 |
Rodriguez-Mejia JL, Hidalgo-Manzano IA, Muriel-Millan LF, Rivera-Gomez N, Sahonero-Canavesi DX, Castillo E, Pardo-Lopez L (2024)
A Novel Thermo-Alkaline Stable GDSL\/SGNH Esterase with Broad Substrate Specificity from a Deep-Sea Pseudomonas sp
Mar Biotechnol (NY)
:
Rodriguez-Mejia JL, Hidalgo-Manzano IA, Muriel-Millan LF, Rivera-Gomez N, Sahonero-Canavesi DX, Castillo E, Pardo-Lopez L (2024)
Mar Biotechnol (NY)
: